collagen 6 antibody  (Thermo Fisher)

Journal: Cell Death Discovery

Article Title: PGC-1α inhibits M2 macrophage polarization and alleviates liver fibrosis following hepatic ischemia reperfusion injury

doi: 10.1038/s41420-023-01636-2

Figure Lengend Snippet: L-02 cells were transfected with PGC-1α activation plasmid ( A , C , E ) or PGC-1α siRNA ( B , D , F ), or control, and treated with 300 μM H 2 O 2 (+) or untreated (−) for 48 h. A , B The levels of IL-6 in the supernatants of cultured hepatocytes were assessed by ELISA. C , D Western blot analysis of the relative levels of JAK2, STAT3, Ym-1 to GAPDH protein expression in RAW264.7 macrophages after co-cultured with the different groups of L-02 cells in transwell plates for 18 h. E , F Immunocytochemistry analysis of Ym-1, Fizz1 protein expression in RAW264.7 macrophages after co-cultured with the different groups of L-02 cells in transwell plates for 18 h. Data represent 4 independent treatments, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Special reagents included antibodies against Collagen I, alpha smooth muscle actin (α-SMA), GAPDH, TNF-α, IL-6, JAK2, STAT3 (Cell Signaling Technology, Beverly, MA, USA); Ym-1 (Abcam, Cambridge, MA, USA), PGC-1α, phospho-NF-kBp65, NF-kBp65, CD204, resistin-like molecule alpha 1 (Fizz1), Flag, CCR2, nitrotyrosine, lipofectamine 2000 and Trizol (Invitrogen, Carlsbad, CA, USA), TSA (Sigma-Aldrich, St Louis, MO, USA), PGC-1α CRISPR activation plasmids (Santa Cruz Biotechnology, Santa Cruz, CA, USA), PGC-1α-specific small interfering RNAs (siRNAs) and negative control siRNAs (Table ) (Oligobio, Beijing, China).

Techniques: Transfection, Activation Assay, Plasmid Preparation, Control, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Immunocytochemistry

Journal: Cell Death Discovery

Article Title: PGC-1α inhibits M2 macrophage polarization and alleviates liver fibrosis following hepatic ischemia reperfusion injury

doi: 10.1038/s41420-023-01636-2

Figure Lengend Snippet: Three days post hepatic I/R, the mice were randomized and injected with the control AAV8 or AAV8-PGC-1α. Their liver tissues were harvested at 4 weeks post hepatic I/R. A H&E, Immunohistochemistry staining of Flag, α-SMA and Sirius red staining. Scale bars: 50 μm. B Western blot analyses of Flag, PGC-1α, IL-6, JAK2, STAT3, Ym-1, Fizz1, CCR2, collagen I and α-SMA expression, # P < 0.05 vs. the Sham-NC, * P < 0.05 vs. the I/R-NC. C RT-qPCR analyses of IL-6, STAT3, Ym-1, Arg-1, collagen I and α-SMA to GAPDH mRNA transcripts. * P < 0.05, ** P < 0.01. D The levels of serum ALT, * P < 0.05. Data are representative images or shown as mean ± SD of each group (6–8/group) from at least three separate experiments.

Article Snippet: Special reagents included antibodies against Collagen I, alpha smooth muscle actin (α-SMA), GAPDH, TNF-α, IL-6, JAK2, STAT3 (Cell Signaling Technology, Beverly, MA, USA); Ym-1 (Abcam, Cambridge, MA, USA), PGC-1α, phospho-NF-kBp65, NF-kBp65, CD204, resistin-like molecule alpha 1 (Fizz1), Flag, CCR2, nitrotyrosine, lipofectamine 2000 and Trizol (Invitrogen, Carlsbad, CA, USA), TSA (Sigma-Aldrich, St Louis, MO, USA), PGC-1α CRISPR activation plasmids (Santa Cruz Biotechnology, Santa Cruz, CA, USA), PGC-1α-specific small interfering RNAs (siRNAs) and negative control siRNAs (Table ) (Oligobio, Beijing, China).

Techniques: Injection, Control, Immunohistochemistry, Staining, Western Blot, Expressing, Quantitative RT-PCR

Journal: Cell Death Discovery

Article Title: PGC-1α inhibits M2 macrophage polarization and alleviates liver fibrosis following hepatic ischemia reperfusion injury

doi: 10.1038/s41420-023-01636-2

Figure Lengend Snippet: The sequences of RNA and DNA oligonucleotides.

Article Snippet: Special reagents included antibodies against Collagen I, alpha smooth muscle actin (α-SMA), GAPDH, TNF-α, IL-6, JAK2, STAT3 (Cell Signaling Technology, Beverly, MA, USA); Ym-1 (Abcam, Cambridge, MA, USA), PGC-1α, phospho-NF-kBp65, NF-kBp65, CD204, resistin-like molecule alpha 1 (Fizz1), Flag, CCR2, nitrotyrosine, lipofectamine 2000 and Trizol (Invitrogen, Carlsbad, CA, USA), TSA (Sigma-Aldrich, St Louis, MO, USA), PGC-1α CRISPR activation plasmids (Santa Cruz Biotechnology, Santa Cruz, CA, USA), PGC-1α-specific small interfering RNAs (siRNAs) and negative control siRNAs (Table ) (Oligobio, Beijing, China).

Techniques: